Antibacterial Activities and Chemical Compounds of Plantago lanceolata (Ribwort Plantain) and Plantago major (Broadleaf Plantain) Leaf Extracts

Background: Plantago lanceolata L. (ribwort plantain) and major (broadleaf are widely used in ethnobotanical studies for treating various diseases. This study aims to investigate the antimicrobial activity chemical compounds of these plants. Methods: The leaf extracts P. were fractioned using different solvents. phytochemical screening was carried out by gas chromatography-mass spectrometry (GC-MS) method. antibacterial assessed disc diffusion method, minimum inhibitory concentration (MIC) bactericidal concentrations (MBC) measured microtiter-broth dilution Results: dichloromethane extract showed highest against Salmonella paratyphi (diameter inhibition zone: 18.83 20.00 mm, respectively) at 100 mg/mL concentration. lowest MIC related both plants S. (500 µg/mL). MBC (1000 µg/mL) paratyphi. main bis(2-ethylhexyl) phthalate (41.96%), 1-methoxy-3-(2-hydroxyethyl)nonane (32.69%), bicyclo[3.1.1]heptane, 2,6,6-trimethyl- (1.alpha.,2.beta.,5.alpha.)- (10.45%), cycloheptasiloxane tetradecamethyl- (27.96% 31.33%). eicosane (23.62%), cyclohexasiloxane dodecamethyl- (18.21%), 1-methyl-3-n-propyl-2-pyrazolin-5-one (18.08%), (33.85%), 1,2-benzisothiazole-3-acetic acid, methyl ester (34.26%). Conclusion: Fractionation methanolic can help better isolate active components from properties two may be due presence detected GC-MS.